2.3 Wound-induced hair follicle neogenesis assay
Three-week-old C57BL/6N male mice were allowed to adapt to their new environment for 3 days .1 cm2 full-thickness wounds were generated on the backs of the mice. 20 μl of each drug was applied to the wounds daily for up to 14, 25, or 40 days. All animal experiments were approved by the Institutional Animal Care and Use Committee of Yonsei University.