2.11 Ex vivo vibrissa follicle culture
Mouse vibrissa follicles were isolated from a C57BL/6 mouse. After euthanizing the mouse, anagen follicles were isolated for organ culture under a stereomicroscope (Nikon). The isolated follicles were placed in 500 μl DMEM supplemented with 100 U/ml penicillin/streptomycin (Gibco) and 12.5 μg/ml gentamicin (Gibco) in 24-well plates. The vibrissa follicles were treated with DMSO, KY19382 (5 μM) or MNX (100 μM). The culture medium was changed every 2 days.
Rat vibrissa follicles were isolated from a 21-day-old male Wistar rat (Philpott, Green, & Kealey, 1992). After euthanizing the rat, anagen follicles were isolated under a stereomicroscope (Nikon). The culture medium was changed every 4 days.