3.2 Isolation and identification of PRV strain from the dead goat
To characterize the features of PRV strain from goat, the supernatants of cerebrum specimen from the dead goat were incubated into monolayer PK15 cells for 1 h, and the cells were then harvested upon observing the appearance of over 80% CPEs. Obvious CPEs could be observed less than 16 h post-infection (Fig. 2A ), furthermore, the presence of PRV nucleic acids (namely, PRV-positive) was confirmed by PCR in the supernatants of the first, second, third, fifth, and seventh passages of cells incubated with YNG strain (Fig. 2B ). Additionally, the infectious virions of YNG and YNP strains in Vero cells were specially recognized by mouse monoclonal antibodies against the gB andgE proteins of PRV via IFA (Fig. 2C ).