3.2 Isolation and identification of PRV strain from the dead
goat
To characterize the features of PRV strain from goat, the supernatants
of cerebrum specimen from the dead goat were incubated into monolayer
PK15 cells for 1 h, and the cells were then harvested upon observing the
appearance of over 80% CPEs. Obvious CPEs could be observed less than
16 h post-infection (Fig. 2A ), furthermore, the presence of PRV
nucleic acids (namely, PRV-positive) was confirmed by PCR in the
supernatants of the first, second, third, fifth, and seventh passages of
cells incubated with YNG strain (Fig. 2B ). Additionally, the
infectious virions of YNG and YNP strains in Vero cells were specially
recognized by mouse monoclonal antibodies against the gB andgE proteins of PRV via IFA (Fig. 2C ).