Figure gel electrophoresis detection
a: IMX581 genome and plasmid pQc009 extraction results. 1,2: IMX581 genome; 3-5: pQc009 plasmid; M: DNA Marker DS10000; M3: DNA Marker 3.
b: Homologous repair template amplification and overlapping extension PCR results. 1: upstream homologous arm XI-5-us; 2: Promoter TEF1p; 3: terminator CYC1t; 4: downstream homologous arm XI-5-ds; 7: PeEgt1containing 20bp promoter TEF1p and terminator CYC1t homologous arm; 10: XI-5-us-TEF1p-PeEgt1 -CYC1t-XI-5-ds; M: DNA Marker 3.
Figure Screening results of single-gene engineered strain and SDS-PAGE results
a: Transformation results of PeEgt1 single genetic engineering strain. A: IMX581-PeEgt1engineering strain; B: IMX581 negative control.
b: SDS-PAGE results. 3: IMX581-PeEgt1 ; CK: IMX581 negative control.
Figure HPLC detection results of EGT yield of single-gene engineered strain
a: EGT standard; b: IMX581 control strain; c: MX581-PeEgt1 .
Figure IMX581engineered strains construction and EGT production
Figure EGT synthesis pathway in P. eryngi