Table 1. Concentration of ascorbic acid (ASA), reduced (GSH)
and oxidized (GSSG) glutathione (in nmol g-1 FW)
measured by HPLC-ESI-TOFMS, and glutathione redox ratio %GSSG
[GSSG/(GSH + GSSG)] x 100] in wild type (Col-0), cad2-1,
pad2-1, rax1-1 and cad1-3 Arabidopsis thaliana
treated with 0 and 3 µM Hg for 72 h. Different letters in the same
column denote significant differences between treatments and genotypes
at p < 0.05 (n = 4) |
Table 1. Concentration
of ascorbic acid (ASA), reduced (GSH) and oxidized (GSSG) glutathione
(in nmol g-1 FW) measured by HPLC-ESI-TOFMS, and
glutathione redox ratio %GSSG [GSSG/(GSH + GSSG)] x 100] in wild
type (Col-0), cad2-1, pad2-1, rax1-1 and
cad1-3 Arabidopsis thaliana treated with 0 and 3 µM Hg for 72 h.
Different letters in the same column denote significant differences
between treatments and genotypes at p < 0.05 (n = 4) |
Table 1. Concentration of ascorbic acid (ASA), reduced (GSH)
and oxidized (GSSG) glutathione (in nmol g-1 FW)
measured by HPLC-ESI-TOFMS, and glutathione redox ratio %GSSG
[GSSG/(GSH + GSSG)] x 100] in wild type (Col-0), cad2-1,
pad2-1, rax1-1 and cad1-3 Arabidopsis thaliana
treated with 0 and 3 µM Hg for 72 h. Different letters in the same
column denote significant differences between treatments and genotypes
at p < 0.05 (n = 4) |
Table 1. Concentration
of ascorbic acid (ASA), reduced (GSH) and oxidized (GSSG) glutathione
(in nmol g-1 FW) measured by HPLC-ESI-TOFMS, and
glutathione redox ratio %GSSG [GSSG/(GSH + GSSG)] x 100] in wild
type (Col-0), cad2-1, pad2-1, rax1-1 and
cad1-3 Arabidopsis thaliana treated with 0 and 3 µM Hg for 72 h.
Different letters in the same column denote significant differences
between treatments and genotypes at p < 0.05 (n = 4) |
Table 1. Concentration of ascorbic acid (ASA), reduced (GSH)
and oxidized (GSSG) glutathione (in nmol g-1 FW)
measured by HPLC-ESI-TOFMS, and glutathione redox ratio %GSSG
[GSSG/(GSH + GSSG)] x 100] in wild type (Col-0), cad2-1,
pad2-1, rax1-1 and cad1-3 Arabidopsis thaliana
treated with 0 and 3 µM Hg for 72 h. Different letters in the same
column denote significant differences between treatments and genotypes
at p < 0.05 (n = 4) |
Table 1. Concentration
of ascorbic acid (ASA), reduced (GSH) and oxidized (GSSG) glutathione
(in nmol g-1 FW) measured by HPLC-ESI-TOFMS, and
glutathione redox ratio %GSSG [GSSG/(GSH + GSSG)] x 100] in wild
type (Col-0), cad2-1, pad2-1, rax1-1 and
cad1-3 Arabidopsis thaliana treated with 0 and 3 µM Hg for 72 h.
Different letters in the same column denote significant differences
between treatments and genotypes at p < 0.05 (n = 4) |
Table 1. Concentration of ascorbic acid (ASA), reduced (GSH)
and oxidized (GSSG) glutathione (in nmol g-1 FW)
measured by HPLC-ESI-TOFMS, and glutathione redox ratio %GSSG
[GSSG/(GSH + GSSG)] x 100] in wild type (Col-0), cad2-1,
pad2-1, rax1-1 and cad1-3 Arabidopsis thaliana
treated with 0 and 3 µM Hg for 72 h. Different letters in the same
column denote significant differences between treatments and genotypes
at p < 0.05 (n = 4) |
|
|
|
ASA |
GSH |
GSSG |
%GSSG |
SHOOTS |
Control |
Col-0 |
4777.77a ± 286.32 |
284.33a ± 27.35 |
6.63a ± 2.16 |
2.3 |
|
|
cad2-1 |
5897.23a ± 1719.75 |
37.12b ± 14.31 |
1.22c ± 0.31 |
3.2 |
|
|
pad2-1 |
5413.97a ± 840.92 |
13.49b ± 3.62 |
0.87c ± 0.37 |
6.0 |
|
|
rax1-1 |
5472.31a ± 663.86 |
55.41b ± 3.55 |
3.66b ± 0.56 |
6.2 |
|
|
cad1-3 |
4595.73a ± 291.22 |
312.09a ± 32.47 |
6.10a ± 1.18 |
1.9 |
|
3 µM Hg |
Col-0 |
6232.62a ±
1890.08 |
281.16a ± 43.41 |
9.25a ±
1.33 |
3.2 |
|
|
cad2-1 |
11718.71b ± 1650.06 |
51.41b ± 18.09 |
1.67c ± 0.34 |
3.1 |
|
|
pad2-1 |
11882.27b ± 1126.86 |
21.08b ± 5.80 |
1.37c ± 0.82 |
6.1 |
|
|
rax1-1 |
10728.59b ± 1149.24 |
145.62c ± 26.91 |
6.65a ± 1.48 |
4.4 |
|
|
cad1-3 |
10399.66b ± 1413.69 |
406.59d ± 32.64 |
12.83b ± 1.50 |
3.1 |
ROOTS |
Control |
Col-0 |
1388.48a ± 256.64 |
195.78a ± 34.61 |
7.40a ± 0.99 |
3.6 |
|
|
cad2-1 |
1336.04a ± 49.52 |
44.62cd ± 10.28 |
1.99c ± 0.61 |
4.3 |
|
|
pad2-1 |
1357.04a ± 260.35 |
14.09d ± 1.65 |
0.93c ± 0.33 |
6.2 |
|
|
rax1-1 |
1302.52a ± 25.00 |
55.38c ± 3.49 |
3.71bc ± 1.48 |
6.3 |
|
|
cad1-3 |
1553.45b ± 62.04 |
280.14ab ± 5.50 |
11.19b ± 0.88 |
3.8 |
|
3 µM Hg |
Col-0 |
1276.32a ±
225.05 |
145.56a ± 7.44 |
4.24a ±
0.99 |
2.8 |
|
|
cad2-1 |
1796.90b ± 194.19 |
82.48c ± 30.96 |
2.91ab ± 0.23 |
3.4 |
|
|
pad2-1 |
1707.96b ± 125.50 |
63.68c ± 11.38 |
2.92ab ± 0.60 |
4.4 |
|
|
rax1-1 |
1924.59b ± 92.65 |
170.52d ± 18.48 |
6.84a ± 1.52 |
3.9 |
|
|
cad1-3 |
1474.29ab ± 129.65 |
513.82b ± 46.85 |
25.03d ± 0.34 |
4.6 |