Mercury tissue concentration
Organs (approximately 100 mg fresh weight -FW-) were dried at 50 ºC for 72 h, and then digested in 1 mL of digestion mixture (HNO3:H2O2:H2O, 0.6:0.4:1 v:v), using an autoclave (Presoclave-75 Selecta, Barcelona, Spain) at 120ºC and 1.5 atm for 30 min (Ortega-Villasante, Hernández, Rellán-Alvarez, Del Campo, & Carpena-Ruiz, 2007). Digests were filtered, diluted in Type I water to 6 mL, and Hg concentrations were determined by ICP-MS NexION 300 Perkin-Elmer Sciex (San Jose, CA, USA).