MS/MS for HgPC2 complex analysis
For LC-MSn measurements the same HPLC system
described above is used, including chromatographic column and analysis
conditions, but the MSn study was performed using a HCT Ultra
high-capacity ion trap (Buker Daltonics). ESI conditions were as
described previously and to establish the optimum
MSn conditions, standard solutions of
HgPC2 were tested. A volume of 20 µL of standard or
xylem sap samples was injected into the LC system and
MSn analysis were controlled by HyStar v 3.2
software (Bruker Daltonics). MSn experiments
were conducted by selecting the high intensity peak mother ion (740.1m/z for MS, and 536.1 m/z for MS2) at
the retention time of the compound chromatographic peak, and data were
collected in total ion counting mode, acquiring the spectra in the range
100-1100 m/z .