Glutathione reductase in gel activity
GR enzymatic activities were determined in gel after separation of protein extracts using non-denaturing polyacrylamide electrophoresis (Sobrino-Plata et al., 2009). Protein loading was 20 and 10 µg for shoot and root samples, respectively. The staining solution was 250 mM Tris-HCl buffer, pH 7.5, supplemented with 0.2 mg mL-1thizolyl blue tetrazolium bromide, 0.2 mg mL-12,6-dichlorophenol indophenol, 0.5 mM NADPH and 3.5 mM GSSG.