Laboratory Testing
Infants with findings suggestive of congenital infections were submitted
to testing for syphilis (treponemal and reaginic tests) and
toxoplasmosis (IgM and IgG ELISA). Also, blood, saliva, urine and/or
liquor were tested for enterovirus, parvovirus, HHV-6, HSV-I, HSV-II,
and CMV via PCR assays. Infants with CNS abnormalities for whom a blood
sample had been obtained within three months of age were tested for
anti-IgM ZIKV antibodies (ZIKV DetectTM 2.0 IgM
Capture Elisa, InBios International, Inc. Seattle, WA, USA), and
ZIKV-RNA (CDC Trioplex Real-time RT-PCR Assay).