2.2 Diagnostic efficacy of detection of the IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 on COVID-19 by ELISA
The ability of prediction of IL-10, IL-1β, IL-6, MCP-1, TNF-α, IP-10 and IL-4 on COVID-19 were assessed by ROC curve, as Figure 4 shows, the experimental group (COVID-19 patients) were positive, and the control group (other diseases patients and healthy people) were negative, and select the tangent point with the largest Youden’sindex as the cut-off value. When select 2.621, 4.898, 1.730, 19.948, 0.110, 7.083 and 0.085 pg / mL as their cut-off values respectively, AUCs of COVID-19 diagnose by they were 0.735, 0.775, 0.595, 0.821, 0.848, 0.387 and 0.987, respectively. The sensitivity of diagnosis of IL-4, TNF-α, MCP-1, IL-1β, IL-10 and IL-6 were 99.1%, 81.2%, 84.6%, 63.2 %, 65.8 % and 72.6%, and the specificity of they were 98.8%, 93.0%, 69.8%, 95.3%, 89.5% and 64.0%; the sensitivity (parallel experiment) and specificity (series experiment) of combined diagnosis of IL-4 and TNF-α were 99.8% and 99.9%, respectively.