2.6. Analysis of drug sensitivity of the SZ01 strain
The paper diffusion method was used to analyze drug sensitivity of SZ01 based on the NCCLS standard ( Wikler, 2009). The SZ01 strain was inoculated onto brain heart infusion agar and cultured for 24 hours with constant shaking (200r/min) at 28 ℃, then diluted to a 107 CFU/mL bacterial suspension using sterile PBS. A total of 100 μL of bacterial solution was added to MH agar, selected drug sensitive paper was placed on the plate (see the table 2 for paper drug content), cultured bacteria at 28 ℃ for 24 hours and measured the diameter of the bacteriostatic circle.