Statistical analysis
Repeated measures analysis of variance (ANOVAr) with Tukey’s honest
significant different (Tukey ‘s HSD) test was used to estimate the
significant variation among treatment combinations of the leaf
physiological, morphological, and chemical traits among the tree age
stages. Data was tested for normality and homogeneity of variance, and,
if necessary, log10 transformation of the data was done
before analysis. The relationship between physiological, leaf
morphological, and chemical traits was determined using multiple linear
regression with regression equations. All analyzed correlations were
considered significantly different when p< 0.05. All the
statistical analysis was performed using R 3.4.4 (R Development Core
Team).