Expression of cytotoxic granules in pNK cells from the fertile
control and RM group
The total number of pNK cells and the expression of cytotoxic granules
in pNK were measured in the fertile control and RM group. No significant
differences were observed in the percentage of circulating
CD3-CD56+ NK cells (13.4% ± 7.6%vs 14.2% ± 7.1%; Fig. 2A and B), granzyme B+(68.2% ± 11.6% vs 68.8% ± 14.5%, Fig. 2C and D),
granulysin+(66.1% ± 11.4% vs 68.2% ± 14.4%, Fig. 2C and E) and
perforin+ NK cells (62.3% ± 13.8% vs 64.3% ±
15.3%, Fig. 2C and F) between the fertile control and RM group. The
mean fluorescence intensity (MFI) of granzyme B reflecting its
expression level in the pNK cells in the control group was significantly
higher than that in RM group (27318.6 ± 3643.8 vs 23774.4 ±
8991.2, P = 0.04, Fig. 2C and G). However, the MFIs of granulysin
(4756.5 ± 1370.7 vs 5043.5 ± 1578.0, P = 0.54, Fig 2C and
H) and perforin (4454.8 ± 1243.9 vs 4972.4 ± 1426.4, P =
0.27, Fig 2C and I) in the pNK cells was not significantly different
between the two groups.