Detection of the receptors mediating the cytotoxicity of pNK cells by flow cytometry
In order to evaluate the expression of the receptors on the surface of NK cells, the heparinized blood was incubated with antibodies against CD3 (5 µL), CD56 (5 µL), NKp30 (5 µL), NKp46 (5 µL), NKG2D (5 µL). In addition, another aliquot of the peripheral blood was incubated with antibodies against CD3 (5 µL), CD56 (5 µL), CD158a (10 µL), CD158b (10 µL). Both aliquots were incubated for 15 min in the dark, then 1x FACS lysis solution was used to lysis the whole blood. Cells were analyzed by BD FACSCanto II flow cytometer equipped with BD FACSDiva software.