Primary antibodies
|
Kv7.4 / KCNQ4
|
NeuroMab,
cat no 75-082, 1 mg/ml
|
77
|
Hu aa 2-77, clone N4 / 36 IgG
|
1:200
(5 µg/ml)
|
no
|
no
|
mouse
|
|
Kv7.4 / KCNQ4*
|
Abcam, ab65797, lot GR94754, whole serum
|
77
|
N’ domain
|
1:100
not available
|
no
|
no
|
rabbit
|
|
Kv7.5 / KCNQ5*
|
Millipore ABN1372-q2476155; 1 ml/ ml
|
~103
|
human IgG
|
1:100
(10 µg/ml)
|
no
|
no
|
rabbit
|
|
PECAM-1 / CD31
|
Santa Cruz Biotechnology,
Sc-1506, 200 µg/ml
|
130
|
699-727 aa at the C-terminus
|
1:100
(2 µg/ml)
|
-
|
-
|
goat
|
|
SM-α-actin
|
Sigma Aldrich
A2547
|
~42
|
N-terminal
|
(1:100)
not available
|
-
|
-
|
mouse
|
Nuclear labels / cell patency markers |
DAPI, Vectasheild |
Vectorlabs |
- |
nucleic acid |
|
- |
- |
- |
|
propidium iodide (PI) |
Sigma, P4170 |
- |
nucleic acid |
10 nM |
- |
- |
- |
Immuno-histochemistry secondary antibodies
|
Mouse 568
|
Abcam, ab175700, lot GR320062-4, 2 mg/ml
|
-
|
IgG
|
1:100
(20 µg/ml)
|
-
|
-
|
donkey
|
|
Rabbit 546
|
Thermofisher, A-11035
|
-
|
IgG
|
1:100
(20 µg/ml)
|
-
|
-
|
goat
|
|
Rabbit 633
|
Merck, SAB4600132, lot 15C0423, 2 mg/ml
|
-
|
IgG
|
1:100
(20 µg/ml)
|
-
|
-
|
donkey
|
Immuno-cytochemistry secondary antibodies
|
Mouse 488
|
Thermofisher, A21202, 2 mg/mL
|
-
|
IgG
|
1:100
(0.02 mg/ml)
|
1:100
(0.02 mg/ml)
|
-
|
donkey
|
|
Rabbit 568
|
Thermofisher,
A10042, 2 mg/mL
|
-
|
IgG
|
1:100
(0.02 mg/ml)
|
1:100
(0.02 mg/ml)
|
-
|
donkey
|
|
Goat 633
|
Thermofisher,
A21082, 2 mg/mL
|
-
|
IgG
|
1:100
(0.02 mg/ml)
|
1:100
(0.02 mg/ml)
|
-
|
donkey
|
Isotype controls |
Mouse IgG |
ThermoFisher, 10400C |
- |
IgG |
5 mg/ml |
- |
- |
mouse |
|
Rabbit IgG |
ThermoFisher, S31235 |
- |
IgG |
10 mg/ml |
- |
- |
rabbit |
Immunoelectron microscopy secondary antibodies |
5 nm Au anti- rabbit |
Merck, G7277, lot SLB3882V |
- |
IgG |
1:100 |
- |
- |
goat |
|
10 nm Au anti- rabbit |
Merck, G7402 |
- |
IgG |
1:100 |
- |
- |
goat |