Reagent purpose Detail Source Predicted MW, kDa Epitope [used] Peptide availability Peptide availability Raised in
Primary antibodies
Kv7.4 / KCNQ4
NeuroMab, cat no 75-082, 1 mg/ml
77
Hu aa 2-77, clone N4 / 36 IgG
1:200 (5 µg/ml)
no
no
mouse
Kv7.4 / KCNQ4*
Abcam, ab65797, lot GR94754, whole serum
77
N’ domain
1:100 not available
no
no
rabbit
Kv7.5 / KCNQ5*
Millipore ABN1372-q2476155; 1 ml/ ml
~103
human IgG
1:100 (10 µg/ml)
no
no
rabbit
PECAM-1 / CD31
Santa Cruz Biotechnology, Sc-1506, 200 µg/ml
130
699-727 aa at the C-terminus
1:100 (2 µg/ml)
-
-
goat
SM-α-actin
Sigma Aldrich A2547
~42
N-terminal
(1:100) not available
-
-
mouse
Nuclear labels / cell patency markers DAPI, Vectasheild Vectorlabs - nucleic acid - - -
propidium iodide (PI) Sigma, P4170 - nucleic acid 10 nM - - -
Immuno-histochemistry secondary antibodies
Mouse 568
Abcam, ab175700, lot GR320062-4, 2 mg/ml
-
IgG
1:100 (20 µg/ml)
-
-
donkey
Rabbit 546
Thermofisher, A-11035
-
IgG
1:100 (20 µg/ml)
-
-
goat
Rabbit 633
Merck, SAB4600132, lot 15C0423, 2 mg/ml
-
IgG
1:100 (20 µg/ml)
-
-
donkey
Immuno-cytochemistry secondary antibodies
Mouse 488
Thermofisher, A21202, 2 mg/mL
-
IgG
1:100 (0.02 mg/ml) 1:100 (0.02 mg/ml)
-
donkey
Rabbit 568
Thermofisher, A10042, 2 mg/mL
-
IgG
1:100 (0.02 mg/ml) 1:100 (0.02 mg/ml)
-
donkey
Goat 633
Thermofisher, A21082, 2 mg/mL
-
IgG
1:100 (0.02 mg/ml) 1:100 (0.02 mg/ml)
-
donkey
Isotype controls Mouse IgG ThermoFisher, 10400C - IgG 5 mg/ml - - mouse
Rabbit IgG ThermoFisher, S31235 - IgG 10 mg/ml - - rabbit
Immunoelectron microscopy secondary antibodies 5 nm Au anti- rabbit Merck, G7277, lot SLB3882V - IgG 1:100 - - goat
10 nm Au anti- rabbit Merck, G7402 - IgG 1:100 - - goat