Reduced signal transduction of the mutant CHRM1
Muscarinic acetylcholine receptors are involved in signalling pathways related to cAMP and calcium intracellular release. CHRM1 is mainly known to signal through Gq/11 activating phospholipase C that increase IP3-calcium related pathways. However, cAMP signalling has also been associated with CHRM1, although its role is still unknown(Peralta et al., 1988). To detect the function levels of the mutant vs. wild-type, reporter assays were performed in HEK293T cells to detect cAMP- (Figure 2C ) or to detect IP3/Ca2+-associated transcription(Figure 2D ). Both reporters displayed a reduced signalling activation for the mutant compared with wild-type.