Reduced signal transduction of the mutant CHRM1
Muscarinic acetylcholine receptors are involved in signalling pathways
related to cAMP and calcium intracellular release. CHRM1 is mainly known
to signal through Gq/11 activating phospholipase C that increase
IP3-calcium related pathways. However, cAMP signalling
has also been associated with CHRM1, although its role is still
unknown(Peralta et al., 1988). To detect the function levels of the
mutant vs. wild-type, reporter assays were performed in HEK293T cells to
detect cAMP- (Figure 2C ) or to detect
IP3/Ca2+-associated transcription(Figure 2D ). Both reporters displayed a reduced signalling
activation for the mutant compared with wild-type.