Statistics
Data are expressed as mean ± standard error of the mean (SEM). Normality
distribution was tested with Shapiro-Wilk test. Two tailed Student’s
test was used to compare two groups, one-way ANOVA was used to compare
one variable in multiple groups and two-way ANOVA to compare two
variables. Both ANOVA were followed by the corresponding post hoc
analyses test. To analyze no-normal distribution data a non-parametric
Mann-Whitney or Kruskal-Wallis tests were used. Survival data were
analyzed using the log-rank test and aneurysm incidence by the Fisher
exact test. GraphPad Prism 8.0 (GrahPad Software, San Diego California
USA) was used to perform the statistical analysis. Values of p
<0.05 were considered statistically significant.