Systolic blood pressure measurements
The LE5001 noninvasive blood pressure acquisition system (Panlab, Hardvard apparatus) and the appropriated cuff and transducer (76-0432 for mice; Panlab Hardvard Apparatus) were used as previously described (Orejudo et al., 2020). Measurements were done in a quiet and temperature regulated area (+/-22° C), being animals preheated (37° C, 10 minutes) before measurements and maintained at 35° C, and having 10 to 15 measurements per animal in each session. Systolic blood pressure is expressed as the mean of 5 to 10 measurements each day, and graphed for the day before first tamoxifen injection, the day of Ang II-osmotic minipumps’ surgery and 7 to 15 days since Ang II systemic administration.