Systolic blood pressure measurements
The LE5001 noninvasive blood pressure acquisition system (Panlab,
Hardvard apparatus) and the appropriated cuff and transducer (76-0432
for mice; Panlab Hardvard Apparatus) were used as previously described
(Orejudo et al., 2020). Measurements were done in a quiet and
temperature regulated area (+/-22° C), being animals preheated (37° C,
10 minutes) before measurements and maintained at 35° C, and having 10
to 15 measurements per animal in each session. Systolic blood pressure
is expressed as the mean of 5 to 10 measurements each day, and graphed
for the day before first tamoxifen injection, the day of Ang II-osmotic
minipumps’ surgery and 7 to 15 days since Ang II systemic
administration.