Figure
legends
Figure 1: Geographic distribution of dogs with confirmed
parvoviral enteritis, mapped according to regions defined by the
Australian Government Department of Infrastructure, Transport, Cities
and Regional Development.
Figure 2: Phylogenetic analysis of the VP2 region of Canine
parvoviruses detected in this study, performed in MEGA X (Kumar 2018).
Evolutionary history was inferred by using the Maximum Likelihood method
and Tamura 3-parameter model with 1000 bootstrap replicates (Tamura,
1992). The tree with the highest likelihood (-3865.87) was selected.
Bootstrap values >70% are shown on the branches. Initial
tree(s) for the heuristic search were obtained automatically by applying
Neighbour-Join and BioNJ algorithms to a matrix of pairwise distances
estimated using the Maximum Composite Likelihood (MCL) approach, and
then selecting the topology with superior log likelihood value. A
discrete Gamma distribution was used to model evolutionary rate
differences among sites (5 categories [+G parameter =
0.5902]). The rate variation model allowed for some sites to be
evolutionarily invariable ([+I ], 84.05% sites). Mink
enteritis virus (MEV) was used as the outgroup. The scale bar indicates
the number of substitutions per site.
Figure 3. Amino acid residues of the capsid protein (VP2) that
differ between: (i) Feline parvovirus (FPV) (in blue) and Canine
parvovirus 2 (CPV-2) (in brown), (ii) CPV-2 and CPV-2a (in green) and
(iii) CPV-2a and CPV-2b or CPV-2c (green). Reverse mutations to FPV are
indicated in blue font and to CPV2 in brown font. The amino acid residue
mutations identified among 21 strains of CPV-2a and CPV-2b (clade 2 of
this study, or FPV-like CPVs) are indicated using a font colour that
matches the different viruses, FPV (blue), CPV-2 (brown) and CPV-2a, 2b
and 2c (green). The frequency of mutations at each position is indicated
(#1 to #21).