FIGURES CAPTIONS
Fig. 1. Experimental sampling design.
Fig. 2. MOTUs richness across the different treatments (control:
immediate extraction; T2: extraction after 6h at room temperature; T3:
extraction after three days at 4°C; T4: immediate preservation in tubes
with silica gel, extraction after 21 days; T5: preservation in tubes
with silica gel after 6h at room temperature, extraction after 21 days)
before (left) and after (right) removing MOTUs with frequency
< 1% in one sample.
Fig. 3. Plots of non-metric dimensional scaling showing dissimilarities
of communities among the three habitats: broadleaved forest (black);
grassland (green); vegetated riverbank (blue). Each plot shows the
results of metabarcoding analysis based on soil samples subjected to
five different treatments.
Fig. 4. Procrustes correlation between communities obtained from
metabarcoding analyses based on soil samples across environmental
conditions subjected to five sample treatments (control: immediate
extraction; RT+6h: extraction after 6h at room temperature; 4°C:
extraction after three days at 4°C; silicagel: immediate preservation in
tubes with silica gel, extraction after 21 days; silicagel+6h:
preservation in tubes with silica gel after 6h at room temperature,
extraction after 21 days. All correlation coefficients are highly
significant (all P < 0.0001).
Fig. 5. Guidelines for improving monitoring strategies with eDNA from
soil.