2.3 Vaccine formulations and animal treatment
Adjuvanted PC:Chol:DOTAP:LPS:Manα liposome (Man-L) was included as follows.
In mice: vaccines were formulated using 15 µg dose of a secreted gD coding plasmid (pCIgD) according to previous dose–response results (Langellotti et al., 2011) or plasmid without gD insert (pCIneo) as negative control. Groups of BALB/c (n=5) were intradermally (i.d.) inoculated at 0 and 20 days with 15 µg /0.3 mL of pCIgD or pCIneo alone; 15 µg /0.3 ml of pCIgD-Man-L or pCIneo-Man-L.
In cattle: a dose of 600 µg pCIgD was chosen based on a dose–response curve (data not shown). Seronegative bovines to BoHV-1 (1–2 years old) were vaccinated i.d. in the back of the ear with 600 µg/1,5 mL (distributed in five sites) of pCIgD (n = 5), pCIgD-Man-L (n = 5) and pCIneo as negative control (n = 4). Bovines were vaccinated at 0, 30 and 60 days.