Identifying responsible chromosomes of ‘Blue’, ‘Yellow’, ‘Spot’
and ‘Red cocoon’ phenotypes in BC1 individuals
BC1 individuals, which has any one of four morphological
traits, ‘Blue,’ ‘Yellow,’ ‘Spot’ and ‘Red cocoon,’ were picked up in
order to identify responsible chromosomes for each trait. Genetic
markers designed for scaffold linkage analysis were utilized (see Table
S4). DNeasy Blood and Tissue kit (QIAGEN) and MightyAmp™ DNA Polymerase
Ver.3 (TaKaRa) was used for DNA extraction and genomic PCR of
BC1 individuals, respectively. The genomic PCR program
was as follows: 2 min at 98°C and 40 cycles of 10 s at 98°C, 15 s at
60°C and 1 min at 68°C.