RNA sequencing (RNA-seq)
Embryo-derived libraries for RNA-seq were prepared using TruSeq RNA
Library Prep Kit (Illumina) and were sequenced using the Illumina HiSeq
2500 platform with 100-bp and 101-bp paired-end reads. The library for
midgut-derived RNA samples was prepared using TruSeq RNA Library Prep
Kit (Illumina) and sequenced using the Genome Analyser IIx System with
76-bp paired-end reads. The library for anterior silk gland- and middle
silk gland-derived RNA samples were prepared using SureSelect Strand
Specific RNA Library Prep Kit (Agilent) and were sequenced using the
Illumina HiSeq 2500 platform with 100-bp paired-end reads. Table S3
summerizes information on the results of RNA-seq.