2.3.3 Histopathological examination
The organs (brain, heart, thymus, lungs, liver, stomach, spleen, kidney, adrenal gland, pancreas and sex organs) excised from all the experimental rats were fixed in 10% formalin in labeled bottles, and processed for histological examination. Tissues embedded in paraffin wax were sectioned 5 mm thick and stained with haematoxylin and eosin, mounted on glass slides and examined under a standard light microscope(10).
2.4 Statistical analysis : All the data was expressed as Mean ± SEM. Statistical analysis was tested by using One-way ANOVA followed by (Dunnett’s test) using Graph pad prism version-8.The significance level was set at P>0.05 for all tests. Group II, III, and IV will be statistically compared with Group I to find the treatment related effects.