Quantitative PCR
Total RNA was extracted using TRIzol reagent (Invitrogen), and 1 μg of total RNA was reverse-transcribed into cDNA using a FastQuant RT Kit (with gDNase) (Tiangen Biotech). RT-PCR was carried out using the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) with Talent qPCR PreMix (SYBR Green) (Tiangen Biotech). Data were normalized to the expression of GAPDH. The primer nucleotide sequences for PCR were obtained from GenBank database and synthesized by Sangon Biotech (China). The primer sequence information used in the study was shown in the supplementary material, Table1.