CXCL2 promoted migration of CD14+ monocytes from RA patients
Flow cytometry analysis using anti-CXCR2 antibody revealed that CD14+ monocytes from ACPA+ patients expressed CXCR2 at a higher level than those from ACPA- patients, but the difference was not statistically significant (Fig. 3A). In the transwell experiments, CXCL2 significantly increased the number of migrating monocytes in cultures derived from RA patients (Fig. 3B).