Flow cytometry
To identify the expression of C-X-C motif chemokine receptor 2 (CXCR2), CD14+ monocytes were incubated for 45 min at 4°C with anti-human CXCR2 in the dark. Cells were washed twice with staining wash buffer and centrifuged at 1000 rpm for 5 minutes to pellet the cells. Stained cells were resuspended in 200 µl of PBS then analyzed via flow cytometry. In each case, staining was compared with that of the appropriately labeled isotype control antibody.