CXCL2 stimulated osteoclastogenesis via ERK MAPK and NFκB pathways
To explore the signal pathways involved in the osteoclastogenesis with the stimulation of CXCL2, RAW264.7 cells were incubated in osteoclast-promoting medium with or without CXCL2, western blotting at different time-points was conducted to detect the phosphorylation of IκBα, p65, ERK1/2 and JNK. The addition of CXCL2 dramatically promoted phosphorylation of p65 and ERK1/2 (Fig. 6A, B), with no effect on the phosphorylation of IκBα or JNK (Fig. 6C, D).