Figure 3: Treg suppression resistant total Teff cells
and Treg suppression sensitive HLA-DR- Teff cells have distinct
expression patterns with respect to certain cytokines and cell surface
receptors. Total Teff and HLA-DR- Teff were sorted by flow cytometry
from 5 pulmonary TB patients. Cells were activated for different times
with anti-CD3/CD28 mitogenic beads, RNA was isolated and an RNA-Seq was
performed. Expression at each time was compared to baseline unactivated
control to arrive at a DEG list. A final DEG list was prepared applying
a cutoff of log2 fold change +/-2.5 and p < 0.05 (for details
on procedure and complete DEG list please see Ahmed A et al., 2018). The
DEG list was further mined to study expression of cytokines, chemokines,
activation markers, cell surface markers and transcription factors. A
summary of these results is shown. The numbers in boxes denote log2 fold
change for expression at 2, 24 and 96 hrs compared to expression at
baseline. For details of the complete DEG list, kindly see [61]