Figure 3: Treg suppression resistant total Teff cells and Treg suppression sensitive HLA-DR- Teff cells have distinct expression patterns with respect to certain cytokines and cell surface receptors. Total Teff and HLA-DR- Teff were sorted by flow cytometry from 5 pulmonary TB patients. Cells were activated for different times with anti-CD3/CD28 mitogenic beads, RNA was isolated and an RNA-Seq was performed. Expression at each time was compared to baseline unactivated control to arrive at a DEG list. A final DEG list was prepared applying a cutoff of log2 fold change +/-2.5 and p < 0.05 (for details on procedure and complete DEG list please see Ahmed A et al., 2018). The DEG list was further mined to study expression of cytokines, chemokines, activation markers, cell surface markers and transcription factors. A summary of these results is shown. The numbers in boxes denote log2 fold change for expression at 2, 24 and 96 hrs compared to expression at baseline. For details of the complete DEG list, kindly see [61]