Supplementary material
Figure S1. Scatterplots between per-sample richness of
taxonomic units from all metabarcoding treatments.
Figure S2. Venn diagrams of the diatom genera relations between
pipelines (ESVs, 97% OTUs, 95% OTUs).
Figure S3 . Scatterplots between per-sample richness of
taxonomic units from all metabarcoding treatments and microscopy.
Figure S4 . Scatterplots between relative abundance of genera
from metabarcoding treatments and microscopy. Relative abundance is
calculated as based on the number of sequences and valves of the
matching genera between metabarcoding and microscopy data sets,
respectively.
Figure S5 . Most important community assembly variables as
selected by Random Forest analyses. Graphs a-d) represent analyses
results for 11 corresponding samples from microscopy and HTS 0.5 data
sets. Graphs from e) to h) represent 14 corresponding samples from
microscopy and HTS 10 data sets.
Table S1. Sediment samples used in this study, and their
measured environmental parameters.
Table S2. Primer used in this study. PCR primers =
adapter+tag+pad+link+primer.
Table S3. ESV/OTU (and genus level) occurrence tables per
samples for sequence data, and species occurrence table for microscopy
data.
Table S4. Marginal tests for all data sets. Included variables
are the ones selected as most important by Random Forest analyses.