2.13. Histology
Tissues were fixed in 10% neural buffered formalin for 24 h. After
dehydration, the tissues were embedded in paraffin, cut into 5 µm thick
sections, and stained with hematoxylin and eosin (H&E). To determine
histopathologic features, digital images were obtained using LAS
microscope software (Leica Microsystems, Wetzlar, Germany).