2.8. Enzyme-linked immunosorbent assay (ELISA)
Samples were prepared from mice serum, BALF supernatant, and cell culture media. Total IgE, OVA-specific immunoglobulins (IgE, IgG1, and IgG2a), cytokines (IFN-γ, TNF-α, IL-4, IL-5, IL-13 and IL-17), histamine, prostaglandin D2 (PGD2) and leukotrienes (LTs, LTB4 and LTC4) were determined using an ELISA kit according to the manufacturer’s protocol. Kits were obtained from Invitrogen, Cayman (Ann Arbor, MI, USA), BioLegend (San Diego, CA, USA), R&D Systems (Minneapolis, MN, USA), and Abcam (Cambridge, UK), respectively. Absorbance was measured using a Victor Nivo Multimode Microplate Reader (PerkinElmer, Boston, MA, USA).