2.14. Cell viability
Cells were seeded in 96-well plates and incubated in culture medium overnight. Cells were then further incubated with either vehicle alone or various concentrations of sophoricoside for 24-72 h. Cell viability was measured at 450 nm using a Victor Nivo Multimode Microplate Reader (PerkinElmer) after further incubation for 2-4 h at 37°C followed by addition of 10 µL CCK-8 Reagent (Daeil Lab Service, Seoul, Korea).