2.8. Enzyme-linked immunosorbent assay (ELISA)
Samples were prepared from mice serum, BALF supernatant, and cell
culture media. Total IgE, OVA-specific immunoglobulins (IgE, IgG1, and
IgG2a), cytokines (IFN-γ, TNF-α, IL-4, IL-5, IL-13 and IL-17),
histamine, prostaglandin D2 (PGD2) and
leukotrienes (LTs, LTB4 and LTC4) were
determined using an ELISA kit according to the manufacturer’s protocol.
Kits were obtained from Invitrogen, Cayman (Ann Arbor, MI, USA),
BioLegend (San Diego, CA, USA), R&D Systems (Minneapolis, MN, USA), and
Abcam (Cambridge, UK), respectively. Absorbance was measured using a
Victor Nivo Multimode Microplate Reader (PerkinElmer, Boston, MA, USA).