Figure
1 Site-mutations influenced binding properties of the ligand
with MOR
A~C. Details of the binding modes of (A) Morphine (67.1
ns), (B) Fentanyl (46.2 ns), (C) Sufentanil (66.4 ns)
D. Radioligand [3H]Fentanyl saturation binding of
wild-type and mutant MOR
E. Fluorescent ligand naltrindole saturation binding of wild-type and
mutant MOR
F,G. HTRF competition binding of (F) Wild-type and (G) W6.48L mutant MOR
All data in the curves are shown as mean ± SEM of three or more assays
run in duplicate, the Ki values were listed inTable 1 .