Figure S5 Functional
activity of test agonists to stimulate MOR-mediated G-protein-dependent
signaling and β-arrestin-dependent signaling
A.B. Remifentanil and 3-methyfentanyl inhibited forskolin-stimulated
cAMP accumulation in (A) wild-type and (B) W6.48L mutant MOR.
C.D. Inhibition of forskolin-stimulated cAMP accumulation in (C) D3.32A
and (D) H6.52L mutant MOR.
E.F. Remifentanil and 3-methyfentanyl induced β-arrestin 2
translocations in (E) wild-type and (F) W6.48L mutant MOR.
G.H. β-arrestin 2 translocations in (G) wild-type and (H) W6.48L mutant
MOR.
All data in the curves are shown as mean ± SEM of three or more assays
run in triplicate. The efficacy and potency parameters were listed in