Figure
2. W6.48L mutation influenced ligand bias to stimulate MOR-mediated
signaling
A, B. Inhibition of forskolin-stimulated cAMP accumulation in (A)
wild-type and (B) W6.48L mutant MOR.
C, D. β-arrestin 2 translocations in (C) wild-type and (D) W6.48L mutant
MOR.
E. The bias parameter ΔΔ log (τ/KA) quantified through
operational model analysis.
All data in the curves are shown as mean ± SEM of three or more assays
run in triplicate, the efficacy and potency parameters were listed inTable 1. The Δ log (τ/KA) and ΔΔ log (τ/KA) values were listed
in Table 2.