3.2 Investigation of M1 formation in vitro.
The mechanism of M1 formation was initially explored by in vitrometabolic incubation system. After incubation for 180 min, less than 10% of ningetinib was consumed, mainly in the liver microsomes (Fig 3). A small amount of M1 (<5%) was detected in HLMs and even less in HIMs. M1 was not detected in HKMs and HLUMs.