2.8. Scanning electron micrographs (SEM)
The
morphological changes in bran before and after extraction were studied
by a Hitachi S-4800 scanning electron microscope system (Hitachi
Corporation, Japan).
Samples
were dried at 105 ℃ for 24 h,
then
were fixed on a specimen holder with an aluminium tape and
sputter-coated with gold. The images were obtained under vacuum with an
acceleration tension of 15 kV.