Pathway 1 Pathway 2 Pathway 3
Transducer (“pathway”) β-arrestin 2 Gi Gq
Cell line CHO CHO CHO
Receptor expression levels (fmol/mg protein) 300 300 300
Time point (min) (For time-resolved, a span)
15-60
30
30
Temperature (°C) 20 20 20
Reference ligand (endogenous) CP55,940 CP55,940 CP55,940
Reference ligand for Emax (if non-endogenous) - - -
Measured process Recruitment Accumulation Accumulation
Measured molecule 1 (a GPCR, transducer, effector or second messenger) CB1 cAMP Myo-inositol-1-phosphate (IP1)
Measured molecule 2 (where applicable) β-arrestin 2
Signal detection technique FRET HTRF HTRF