Data and statistical analysis
The data and statistical analysis comply with the recommendations on
experimental design and analysis in pharmacology
(Curtis et al. , 2018). Since in
microdialysis studies, particularly when amino acid are measured,
baseline values can differ greatly from animal to animal, to minimize
experimental variability data were normalized as percent of baseline
values, as in our previous publications. Statistical analysis was
performed (Graphpad Prism 6.0) on area-under-the curve values
(AUC; calculated in the 100-220 min
range) followed by the Newman-Keuls test for multiple comparisons. AIMs
were statistically analysed using the Mann-Whitney U-test or the
Kruskal-Wallis test followed by the Dunn test for multiple comparisons
when more than two groups were compared. Alpha was set at 0.05.