Materials
L-DOPA methyl ester, benserazide hydrochloride, tropicamide and
VU-0152100 were purchased from Sigma-Aldrich (S. Louis MO, USA), 6-OHDA
hydrobromide, telenzepine, PD-102807 and AFDX-116 from Tocris (Bristol,
UK). Telenzepine, PD-102807, tropicamide, AFDX-116 and VU-0152100 were
dissolved in Ringer for local perfusion through the microdialysis probe.
6-OHDA was dissolved in ascorbate-saline for intracerebral injection,
whereas all the other drugs were dissolved in saline and administered
systemically at the volume of 1.0 ml/Kg body weight.