Genome comparison
Genome synteny was analyzed using ProgressiveMauve (Darling, Mau, &
Perna, 2010). The complete closed genome of the Neisseria -related
symbiont from H. acanthopus was aligned to the 39 contigs of theNeisseria -related symbiont from P. serrata , yielding 27
Locally Collinear Blocks (Figure 1). The resulting synteny regions are
explored in more detail in SupplementaryData1. To provide a comparison
of selected metabolic pathways, we extended the analysis from our
previous work on Legionella polyplacis (Rihova, Novakova, Husnik,
& Hypsa, 2017) which followed the selection of genes published by
Moran, McCutcheon and Nakabachi (2008). The comparison was based on the
RAST annotation for the two Neisseria -related symbionts, and the
KEGG database (Kanehisa, Sato, Kawashima, Furumichi, & Tanabe, 2016)
for our other bacteria.