Materials and Methods
Wistar rats (male, age 2–4 months, weight 200–250 g) were delivered
from Stolbovaya Breeding Farm (Russian Federation) and kept in standard
conditions. Animal care was performed according to the guidelines
established by the European Council Directive 2010/63/EU and in
accordance with the “Regulations for Studies with Experimental
Animals” (Decree of the Russian Ministry of Health of 12 August 1997,
No. 755). The protocol was approved by the Commission on Biological
Safety and Ethics of the Institute of Cell Biophysics, Russian Academy
of Science. The light cycle was 12 h light and 12 h dark. The air
temperature was maintained at about 21 °C to 22 °C. A standard diet and
water were provided ad libitum. All animals were delivered in the
experimental room in no less than two hours before the beginning of the
experiment.