Soil abiotic parameters
Soil samples used to measure soil abiotic characteristics were dried at 40 °C and sieved through a 2 mm sieve. Approximately three grams of dried soil was mixed with 30 ml of 0.01 M CaCl2. The mixture was shaken for 2 h on a mechanical shaker with linear movement at 250 rpm. The samples were centrifuged for 5 m at 3000 rpm. Then, 15 mL of the supernatant was filtered through a Whatman Puradisc Aqua 30 syringe filter with cellulose acetate membrane. Subsequently, 12.87 mL of the filtrate and 130 μL HNO3 were mixed in a clean 15 mL tube. The sample was mixed using a vortex and analyzed for soil extractable micronutrients (Fe, K, Mg, P, S, Zn) using an inductively coupled plasma - optical emission spectrometer (ICP-OES, Thermo Scientific iCAP 6500 Duo Instrument with axial and radial view and CID detector microwave digestion system). The remaining part of the filtrate was transferred to a 15 mL tube to measure soil pH, NO2+NO3 and NH4. After recording the pH (inoLab pH 7310), the soil extracts were analyzed on a QuAAtro Autoanalyzer (Seal Analytical, Mequon, Wisconsin, USA).