Keyword : Imagej Macro Langage
The experiment :
Microalgae are electropored and bathed into a fluorescent dextran solution of different molecular weight. If holes are created and are big enough, dextran molecules will enter the microalgae and fluorescence will be measured. Acquisition is performed on a confocal microscope. Single plane is acquired (at the center of the microalgae).
We acquire different channel : Dextran (green fluorescence), Membrane (tagged in yellow fluorescence XXX), Chlorophyll (Autofluorescence in red), visible channel (for visualization of microalgae integrity).