sourcecode : part 1
2. Step 2 : Segmentation
Segmentation occurs in two major steps. Preparation of the MembraneChannel and Segmentation itself.
1. As the membrane labelling can have a noisy background, we realise this series of action
Duplicate the MembraneChannel image
Run a gaussian blur
Realise the substraction of MembraneChannel by this new image we called BackgroundCleanup.
Close the image to save space
Rename the result of subtraction to be called ForSegmentation.