With the development of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems
https://www.cell.com/molecular-cell/fulltext/S1097-2765(18)30880-3
Generation of Single-Cell Transcript Variability by Repression
https://www.cell.com/current-biology/fulltext/S0960-9822(17)30564-X
"Gene expression levels vary greatly within similar cells, even within clonal cell populations [1]. These spontaneous expression differences underlie cell fate diversity in both differentiation and disease [2]. The mechanisms responsible for generating expression variability are poorly understood. "
[1] Raj A. van Oudenaarden A. Nature, nurture, or chance: stochastic gene expression and its consequences.Cell. 2008; 135: 216-226
[2] Eldar A. Elowitz M.B. Functional roles for noise in genetic circuits.Nature. 2010; 467: 167-173
Manifold methods:
https://www.sciencedirect.com/science/article/pii/S2452310017301877