With the development of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems
Generation of Single-Cell Transcript Variability by Repression
"Gene expression levels vary greatly within similar cells, even within clonal cell populations [1]. These spontaneous expression differences underlie cell fate diversity in both differentiation and disease [2]. The mechanisms responsible for generating expression variability are poorly understood. "
[1] Raj A.
van Oudenaarden A.
Nature, nurture, or chance: stochastic gene expression and its consequences.Cell. 2008; 135: 216-226
[2] Eldar A.
Elowitz M.B.
Functional roles for noise in genetic circuits.Nature. 2010; 467: 167-173
Manifold methods: