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CRISPR-Cas systems in Serratia
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  • Maria Scrascia,
  • Roberta Roberto,
  • Pietro Daddabbo,
  • Yosra Ahmed,
  • Francesco Porcelli,
  • Marta Oliva,
  • Carla Calia,
  • Angelo Marzella,
  • Carlo Pazzani
Maria Scrascia
University of Bari
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Roberta Roberto
University of Bari
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Pietro Daddabbo
University of Bari
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Yosra Ahmed
Plant Pathology Research Institute
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Francesco Porcelli
University of Bari
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Marta Oliva
University of Bari
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Carla Calia
University of Bari
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Angelo Marzella
University of Bari
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Carlo Pazzani
University of Bari
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Abstract

The CRISPR-Cas system of Prokaryotes is an adaptive immune defense mechanism to protect themselves from invading genetic elements (e.g. phages and plasmids). Studies that describe the genetic organization of these prokaryotic systems have mainly reported on the Enterobacteriaceae family (now reorganized within the order Enterobacteriales). For some genera, data on CRISPR-Cas systems remain poor, as in the case of Serratia (now part of the Yersiniaceae family) where data are limited to a few genomes of the species marcescens. This study describes the detection, in silico, of CRISPR loci in 146 Serratia complete genomes and 336 high-quality assemblies available for the species ficaria, fonticola, grimesii, inhibens, liquefaciens, marcescens, nematodiphila, odorifera, oryzae, plymuthica, proteomaculans, quinivorans, rubidaea, symbiotic, and ureilytica. Apart from subtypes I-E and I-F1, which had previously been identified in marcescens, we report that of I-C and the variants I-ES1, I-ES2 and I-F1S1. Analysis of the genomic contexts for CRISPR loci revealed mdtN-phnP as the region mostly shared (grimesii, inhibens, marcescens, nematodiphila, plymuthica, rubidaea, and Serratia sp.). Three new contexts detected in genomes of rubidaea and fonticola (puu genes-mnmA) and rubidaea (osmE-soxG and ampC-yebZ) were also found. Plasmid and/or phage origin of spacers was also established.
26 Jul 2022Submitted to MicrobiologyOpen
29 Jul 2022Assigned to Editor
29 Jul 2022Submission Checks Completed
04 Aug 2022Reviewer(s) Assigned
31 Aug 2022Review(s) Completed, Editorial Evaluation Pending
31 Aug 2022Editorial Decision: Revise Minor