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Comparative Analysis of the Vaginal Microbiome Between women with Polycystic ovary syndrome and Healthy women: A Large-sample cross-sectional study
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  • Congcong Jin,
  • Lang Qin,
  • Zheng Liu,
  • Xiao Li,
  • Xuan Gao,
  • Yongzhi Cao,
  • Shigang Zhao,
  • Jiaojiao Wang,
  • Ting Han,
  • lei yan,
  • Jialun Song,
  • Fangfang Zhang,
  • Feifei Liu,
  • Yousheng Zhang,
  • Yuzhen Huang,
  • Yuping Song,
  • Yanjun Liu,
  • Zhina Yao,
  • Honglei Chen,
  • Zhenzhen Zhang,
  • Shengrui Zhao,
  • Yuhan Feng,
  • Yanan Zhang,
  • Qian Yu,
  • Tianyong Sun,
  • Qing Feng,
  • Han Zhao
Congcong Jin
Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China
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Lang Qin
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Zheng Liu
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Xiao Li
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Xuan Gao
1.Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yongzhi Cao
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Shigang Zhao
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Jiaojiao Wang
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Ting Han
Center for Reproductive Medicine, Reproductive Hospital Affiliated to Shandong University, Jinan
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lei yan
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Jialun Song
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Fangfang Zhang
1.Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Feifei Liu
1.Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yousheng Zhang
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yuzhen Huang
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yuping Song
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yanjun Liu
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Zhina Yao
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Honglei Chen
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Zhenzhen Zhang
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Shengrui Zhao
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yuhan Feng
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Yanan Zhang
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Qian Yu
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Tianyong Sun
Department of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.
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Qing Feng
Department of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.
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Han Zhao
Center for Reproductive Medicine, Shandong University, Jinan, Shandong, 250012, China.
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Abstract

Abstract Objectives To investigate the vaginal microbiome (VMB) of a large sample size of polycystic ovary syndrome (PCOS) patients using 16S ribosomal RNA (16S rRNA) sequencing. Design A cross-sectional study Setting Ji Nan, China Sample A total of 1,446 subjects were recruited (PCOS, n=713, the controls, n=733). Methods Vaginal swabs were analyzed using 16S rRNA gene sequencing. Main outcome measures The microbiome diversity and composition of the PCOS group and control group were compared. In the PCOS prediction model, microbial interaction networks and functions prediction were investigated. Results The PCOS group had a higher alpha diversity in the VMB than controls (P<0.05), while higher intra-group variability was observed in PCOS (P<0.05). At the genus level, the proportion of Lactobacillus in the PCOS group decreased, while the proportion of Gardnerella and Ureaplasma increased (FDR<0.2). Gardnerella vaginalis, Prevotella buccalis, and Prevotella timonensis were identified as differential species and were strongly associated with blood parameters of PCOS. The VMB interaction network indicated that Prevotella and Lactobacillus may be key drivers in the PCOS group. Overall, 55 differentially predicted genes were found between PCOS and controls (FDR<0.25). Conclusions The PCOS group had a higher diversity in the vaginal microbiome and showed an enhanced level of heterogeneity. The proportion of Lactobacillus in PCOS group decreased, while the proportions of Gardnerella and Ureaplasma increased. These results warrant further research that can validate the correlation between PCOS and VMB.