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Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb gene sequences
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  • Mahmudul Hasan,
  • Nia Kurniawan,
  • Aris Soewondo,
  • Wilmientje Nalley,
  • Masafumi Matsui,
  • Takeshi Igawa,
  • Masayuki Sumida
Mahmudul Hasan
Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University

Corresponding Author:[email protected]

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Nia Kurniawan
Brawijawa University
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Aris Soewondo
Brawijawa University
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Wilmientje Nalley
University of Nusa Cendana
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Masafumi Matsui
Kyoto University
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Takeshi Igawa
Hiroshima University
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Masayuki Sumida
Hiroshima University
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Abstract

In order to interpret the degree of postmating isolation and the evolutionary relationships among Fejervarya species from Indonesia (Lesser Sunda), Bangladesh, China, and Japan, crossing experiments and molecular phylogenetic analyses were carried out using frogs of Fejervarya species from these countries. The crossing experiments revealed that the reciprocal hybrids among F. iskandari, F. verruculosa, and F. sp. Large type, and those between F. multistriata and F. kawamurai were viable through metamorphosis, but those between F. iskandari group and F. limnocharis group were completely or partially inviable at the tadpole stage, and those between Southeast -Asian and South-Asian Fejervarya groups were completely inviable at the embryonic stage. The matured reciprocal hybrids between F. iskandari and F. verruculosa from Indonesia, Lesser Sunda showed some degree of abnormality in spermatogenesis. The phylogenetic analyses using mtDNA Cytb gene sequences demonstrated that F. iskandari formed a sister clade with F. verruculosa from Lesser Sunda, Indonesia with 8.1% sequence divergence. Fejervaraya multistriata from China made a clade with Thailand, Malaysia and Indonesian (topotype) populations of F. limnocharis which showed sister relationships to F. kawamurai from Japan with 8.9% sequence divergence of Cytb gene. Fejervarya sp. small type from Bangladesh formed a clade with the other South-Asian members of Fejervarya group and made a sister clade with Southeast-Asian Fejervarya group having 23.1% sequence divergence of Cytb gene. This study showed that the degree of postmating isolation reflects the molecular phylogenetic relationships, and that the two species, F. iskandari and F. verruculosa from Indonesia (Lesser Sunda) are reproductively isolated by abnormal spermatogenesis, and genetically deviated.
11 Jun 2022Submitted to Ecology and Evolution
13 Jun 2022Submission Checks Completed
13 Jun 2022Assigned to Editor
21 Jun 2022Reviewer(s) Assigned
07 Jul 2022Review(s) Completed, Editorial Evaluation Pending
07 Jul 2022Editorial Decision: Revise Minor
06 Sep 20221st Revision Received
06 Sep 2022Assigned to Editor
06 Sep 2022Submission Checks Completed
06 Sep 2022Review(s) Completed, Editorial Evaluation Pending
09 Sep 2022Editorial Decision: Revise Minor
19 Sep 20222nd Revision Received
19 Sep 2022Submission Checks Completed
19 Sep 2022Assigned to Editor
19 Sep 2022Review(s) Completed, Editorial Evaluation Pending
27 Sep 2022Editorial Decision: Revise Minor
28 Sep 20223rd Revision Received
29 Sep 2022Assigned to Editor
29 Sep 2022Submission Checks Completed
29 Sep 2022Review(s) Completed, Editorial Evaluation Pending
29 Sep 2022Editorial Decision: Accept
Oct 2022Published in Ecology and Evolution volume 12 issue 10. 10.1002/ece3.9436