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Detection of antimicrobial resistance genes in urban air
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  • Ágnes Becsei,
  • Norbert Solymosi,
  • István Csabai,
  • Donát Magyar
Ágnes Becsei
Eötvös Loránd Tudományegyetem
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Norbert Solymosi
University of Veterinary Medicine Budapest Hutÿra Ferenc Library Archives and Museum
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István Csabai
Eötvös Loránd Tudományegyetem
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Donát Magyar
National Center for Public Health
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Abstract

To understand antibiotic resistance in pathogenic bacteria, we need to monitor environmental microbes as reservoirs of antimicrobial resistance genes (ARGs). These bacteria are present in the air and can be investigated with the whole metagenome shotgun sequencing approach. This study aimed to investigate the feasibility of a method for metagenomic analysis of microbial composition and ARGs in the outdoor air. Air samples were collected with a Harvard impactor in the PM10 range at 50 m from a hospital in Budapest. From the DNA yielded from samples of PM10 fraction single-end reads were generated with an Ion Torrent sequencer. During the metagenomic analysis, reads were classified taxonomically. The core bacteriome was defined. Reads were assembled to contigs and the ARG content was analyzed. The dominant genera in the core bacteriome were Bacillus, Acinetobacter, Leclercia and Paenibacillus. Among the identified ARGs best hits were vanRA, Bla1, mphL, Escherichia coli EF-Tu mutants conferring resistance to Pulvomycin, BcI, FosB, and mphM. Despite the low DNA content of the samples of PM10 fraction, the number of detected airborne ARGs was surprisingly high.

Peer review status:Published

23 Aug 2021Submitted to MicrobiologyOpen
25 Aug 2021Submission Checks Completed
25 Aug 2021Assigned to Editor
27 Aug 2021Reviewer(s) Assigned
20 Sep 2021Review(s) Completed, Editorial Evaluation Pending
20 Sep 2021Editorial Decision: Revise Minor
10 Oct 20211st Revision Received
11 Oct 2021Assigned to Editor
11 Oct 2021Submission Checks Completed
11 Oct 2021Review(s) Completed, Editorial Evaluation Pending
11 Oct 2021Reviewer(s) Assigned
13 Oct 2021Editorial Decision: Revise Minor
18 Oct 20212nd Revision Received
19 Oct 2021Assigned to Editor
19 Oct 2021Submission Checks Completed
19 Oct 2021Review(s) Completed, Editorial Evaluation Pending
20 Oct 2021Editorial Decision: Accept
Nov 2021Published in MicrobiologyOpen volume 10 issue 6. 10.1002/mbo3.1248