Differential Expression and Functional Analysis of High-throughput
Sequencing about Long Noncoding RNAs in Corneal Transplantation
Abstract
Background: Immune rejection is still the main cause of transplant
failure of corneal transplantation which mechanism is not fully
understood. The purpose of this study is to investigate the differential
expression of long noncoding RNAs (lncRNAs) in corneal allograft
rejection and to construct a network diagram of the interaction between
lncRNAs and microRNAs(miRNAs). Methods: The lncRNAs expression profile
of rat corneal transplantation was constructed by high throughput
sequencing. The co-expressed mRNA was analyzed by gene ontology (GO),
gene and genomic Kyoto encyclopedia (KEGG). An interaction network
diagram of lncRNAs, miRNA and rejection related target genes was
constructed. Part of the prediction was verified by real-time polymerase
chain reaction (qPCR). Results: A total of 285 lncRNAs expressions were
detected between the normal group and the autograft group, with 239
lncRNAs significantly upregulated and 46 lncRNAs downregulated, while
162 lncRNAs were upregulated and 20 downregulated between the allograft
group and the autograft group. Go and KEGG were used to enrich and
analyze the co-expression of mRNA. By analyzing the interaction between
lncRNAs, miRNAs and target genes related to corneal allograft rejection,
56 upregulated lncRNAs,7 downregulated lncRNAs, 6 upregulated miRNAs and
4 downregulated miRNAs were found in allograft and autograft group.
Three of the possible pathways were confirmed and verified by qPCR .
Conclusions: The results showed that there was a difference in lncRNA
expression between normal ,autograft and allograft group. LncRNAs may be
a new molecular target in the treatment of corneal injury and corneal
allograft rejection by interact with miRNAs.