Distribution and Immunomodulatory mechanisms of Programmed death(PD)-1
molecule , its ligand PD-L1and related cytokines in Cervical Cancer
Abstract
PD1(programmed cell death protein 1), a molecule negatively regulated by
immunity,is highly expressed in a wide variety of human malignancies
making it an attractive immunotherapeutic target. PD-L1 is one of the
ligands for PD-1 and expressed both on T cells and tumor cells. The
purpose of this study was to investigate the expression levels of PD1,
PD-L1, regulatory T cells (Tregs), identified as CD4+CD25+CD127-/low,
and related cytokines during the development and progression of cervical
cancer(CC). Through different groups to simulate the process of the
occurrence and development of CC, peripheral blood of each group was
collected, and different indicators were detected by ELISA and flow
analysis. It was found that except for INF-γ, PD1, PD-L1, IL-2, IL6,
IL-10, TGF-β expression increased gradually with the development of CC,
and the differences between groups were statistically significant
(P<0.05). This situation also appears on the tracking of
preoperative CC patients and postoperative follow-up on test results.
And Tregs in peripheral blood, compared with normal control(NC) group,
the expression increased CC group, the PD1 expression in the Tregs
membrane also increases at the same time. In CC tissues, we found an
interesting phenomenon through multiplex immunohistochemical staining
that CD8+ T cells mainly infiltrated in the tumor cell aggregation area,
while CD4+ T cells mainly appeared around. Therefore, we speculated that
CD8+ T cells mainly played an anti-tumor immune role in CC tumor
tissues.